Activation of NF - k B by FADD , Casper , and Caspase - 8 *

Fas-associated death domain protein (FADD), caspase-8-related protein (Casper), and caspase-8 are components of the tumor necrosis factor receptor type 1 (TNF-R1) and Fas signaling complexes that are involved in TNF-R1and Fas-induced apoptosis. Here we show that overexpression of FADD and Casper potently activates NF-kB. In the presence of caspase inhibitors, overexpression of caspase-8 also activates NF-kB. A caspaseinactive point mutant, caspase-8(C360S), activates NF-kB as potently as wild-type caspase-8, suggesting that caspase-8-induced apoptosis and NF-kB activation are uncoupled. NF-kB activation by FADD and Casper is inhibited by the caspase-specific inhibitors crmA and BD-fmk, suggesting that FADDand Casper-induced NF-kB activation is mediated by caspase-8. FADD, Casper, and caspase-8-induced NF-kB activation are inhibited by dominant negative mutants of TRAF2, NIK, IkB kinase a, and IkB kinase b. A dominant negative mutant of RIP inhibits FADDand caspase-8-induced but not Casper-induced NF-kB activation. A mutant of Casper and the caspase-specific inhibitors crmA and BD-fmk partially inhibit TNF-R1-, TRADD, and TNF-induced NF-kB activation, suggesting that FADD, Casper, and caspase-8 function downstream of TRADD and contribute to TNF-R1-induced NF-kB activation. Moreover, activation of caspase-8 results in proteolytic processing of NIK, which is inhibited by crmA. When overexpressed, the processed fragments of NIK do not activate NF-kB, and the processed C-terminal fragment inhibits TNF-R1-induced NF-kB activation. These data indicate that FADD, Casper, and pro-caspase-8 are parts of the TNF-R1-induced NF-kB activation pathways, whereas activated caspase-8 can negatively regulate TNF-R1-induced NF-kB activation by proteolytically inactivating NIK.